Экспрессия белка относится к синтезу белки within cells using information contained in the DNA or gene.
Белки are responsible for all the biochemical reactions that take place within the cell. Hence, it makes it necessary to study белок function in order to understand cellular processes.
This is currently studied based on using fluorescent белки as tags. However, this does not provide real time analysis as it requires maturation of chromophore that takes time and this causes a delay in investigation of real-time expression, especially for белки преходящие или недолговечные по своей природе.
Исследователи сообщили о новом методе на сервере препринтов 30 июля 2020 года, который может преодолеть это ограничение.
The new study describes the use a fluorescent biosensor that enables real-time detection of белок expression in vivo which has implications for understanding the spatiotemporal expression of белки within the living organism. This sensor is based on a dim green fluorescent белок in which pre-existing fluorescence increases 11-fold in vivo following the specific and rapid binding of a белок tag and enables detection of белок expression within seconds in live cells.
Эта биодатчик will be useful to study biological processes in real-time where белки are either transiently expressed and/or to understand the expression and interactions of белки from a disease-causing organism such as bacteria or a virus with that of the host proteins.
Ссылка:
Исон М.Г., Пандельева А.Т., Майер М.М. и др., 2020. Генетически кодируемый флуоресцентный биосенсор для быстрого обнаружения экспрессии белка. Препринт: bioRxiv 2020.07.30.229633; DOI: https://doi.org/10.1101/2020.07.30.229633
